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Korean J Thorac Cardiovasc Surg 1993; 26(2): 80-85

Published online February 5, 1993

Copyright © Journal of Chest Surgery.

Blood compatability of artificial blood-contacting surface selected with cultured bovine endothelial cells

김원곤,곽영태,유세영

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Synthetic and biosynthetic vascular grafts of small diameter have long been considered to be prone to thrombosis,ultimately Leading to the complete graft occlusion.Endothelial cell seeding onto synthetic blood-contacting surfaces has been suggested to be an ideal means to solve this problem.This study described a culture method of bovine endothelial cells and evaluated blood-compatibility and seeding efficiency of cultured endothelial cells. Bovine pulmonary artery endothelial cells were harvested enzymatically and grown to confluence on polystyrene culture flask surfaces using established techniques.The identification of endothelial cells was made through the demonstration of expression of factor VIII R:Ag by immunofluorescent technique.To quantitate the effect of improvement in blood-compatibility of viable endothelial cells,endothelial monolayers were exposed to blood containing 111In-oxine labeled platelets. Viable endothelial monolayers retained less labeled platelets than control surfaces. The Indium-labeled endothelial cells were seeded onto three different blood-contacting surfaces of Dacron vascular graft immobilized in specially equipped wells and incubated for specific time intervals[t=15,30,60,120 minutes].Longer incubation times showed improved cell adherence in collagen-coated and fibrin-coated Dacron vascular graft groups. However in untreated Dacron grafts,no direct relationship was observed between incubation time and endothelial cell seeding efficiency.This may be due to leakage of endothelial cells through porosity of Dacron grafts in this in-vitro experimental condition.[KTCS 1993;2:80-85]

Article

Korean J Thorac Cardiovasc Surg 1993; 26(2): 80-85

Published online February 5, 1993

Copyright © Journal of Chest Surgery.

Blood compatability of artificial blood-contacting surface selected with cultured bovine endothelial cells

김원곤,곽영태,유세영

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Synthetic and biosynthetic vascular grafts of small diameter have long been considered to be prone to thrombosis,ultimately Leading to the complete graft occlusion.Endothelial cell seeding onto synthetic blood-contacting surfaces has been suggested to be an ideal means to solve this problem.This study described a culture method of bovine endothelial cells and evaluated blood-compatibility and seeding efficiency of cultured endothelial cells. Bovine pulmonary artery endothelial cells were harvested enzymatically and grown to confluence on polystyrene culture flask surfaces using established techniques.The identification of endothelial cells was made through the demonstration of expression of factor VIII R:Ag by immunofluorescent technique.To quantitate the effect of improvement in blood-compatibility of viable endothelial cells,endothelial monolayers were exposed to blood containing 111In-oxine labeled platelets. Viable endothelial monolayers retained less labeled platelets than control surfaces. The Indium-labeled endothelial cells were seeded onto three different blood-contacting surfaces of Dacron vascular graft immobilized in specially equipped wells and incubated for specific time intervals[t=15,30,60,120 minutes].Longer incubation times showed improved cell adherence in collagen-coated and fibrin-coated Dacron vascular graft groups. However in untreated Dacron grafts,no direct relationship was observed between incubation time and endothelial cell seeding efficiency.This may be due to leakage of endothelial cells through porosity of Dacron grafts in this in-vitro experimental condition.[KTCS 1993;2:80-85]

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